Proteomics is taking off with Genomics Solutions, Pharmacia, Micromass, PE
etc offering 'complete packges' anywhere from gel running through to ESItof
(some packages real, some due for 2000 launch). Central to the successful
operation of these setups must be an automated in-gel digestion system
whose ability to deliver on low abundance samples must match or exceed that
achievable through 'dirty' manual means.
My question: who has experience of automated digesters and possibly
automated clean-up devices/spotters? Are they any good? Are they useful at
the sensitivity needed to identify Ag-stained spots?
Any feedback welcome, especially on quantitation aspects.
Len
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Dr Len C. Packman
Assistant Director of Research
Protein and Nucleic Acid Chemistry Facility
Department of Biochemistry
University of Cambridge
80 Tennis Court Road
Old Addenbrookes Site
Cambridge, CB2 1GA, UK
Tel: +44 (1223) 333639
FAX: +44 (1223) 766002
e-mail: lcp2@mole.bio.cam.ac.uk
Visit my WWW page at http://www.bio.cam.ac.uk/proj/adr/PNAC/pnac.html