A possible source for labelled amino acids is Cambridge Isotopes.
There are basically 2 procedures for Fmoc acylation of amino acids:
using Fmoc chloride: J. Meienhofer et al. Int. J. Peptide Protein
Res. 13, 1979, 35-42
or by using Fmoc succinimide: G.F. Sigler, M. Goodman et. al.
Biopolymers, Vol 22 2157-2162
The Fmoc succinimide is the "milder" procedure. I prefer it. The key
issue is to precipitate the protonated Fmoc amino acid after the
acylation, ether and ethylacetate extractions. Most amino acids
precipitate (which means easy isolation and work-up) but very
hydrophilic (unusual) amino acids may not.
Then it may become difficult and tedious to isolate/purify the Fmoc
amino acid (I did it once by gelfiltration).
Good luck!
Henriette
>Hey Folks-
>
>If I want to make a radioactive peptide (no iodinatable residues)
>using tritium or carbon-14 :
>
>- I can buy radioactive amino acids, but are the radioactive FMOC amino acids?
>
>- I know BOC-On, but is there FMOC-On? i.e., how do I put on the
>FMOC group if I need to?
>
>Tom Andersen
>
>
>ÿ-------------------------
>Thomas T. Andersen, Ph.D.
>Protein Chemistry Core Facility
>Albany Medical College
>Albany, New York
Henriette A. Remmer, Ph.D
Director
Protein Sciences Facility
Biotechnology Center
University of Illinois
311/315 Noyes Laboratory, Box 62-1
505 S. Mathews Ave.
Urbana,IL 61801
Tel: (217) 333-4695 (Lab) (217) 333-3841 (office)
Fax: (217) 244-1142
homepage: http://www.life.uiuc/biotech/protein_sc.html
-------------------------------------