If so, what are the currently preferred methodologies?
I see Kent has a company (http://www.gryphonsci.com/) doing this now.
Anyone use his approach of ligating unprotected peptides? Does it work with
hydrophobic proteins (say, 2x40mer--> 80mer)
Anyone use the approach of ligating Hmb-protected peptides?
Len
*********************************************************************
Dr Len C. Packman
Assistant Director of Research
Protein and Nucleic Acid Chemistry Facility
Department of Biochemistry
University of Cambridge
80 Tennis Court Road
Old Addenbrookes Site
Cambridge, CB2 1GA, UK
Tel: +44 (1223) 333639
FAX: +44 (1223) 766002
e-mail: lcp2@mole.bio.cam.ac.uk
Visit my WWW page at http://www.bio.cam.ac.uk/proj/adr/PNAC/pnac.html