At 12:37 16/11/99 +0000, you wrote:
>Dea ABRFers
>
>Only one response to my robotics question so far. Has no-one used these
>instruments or is there shyness in commenting (you can always use the
>ombudsman). As a forum we should feel free to name vendors where
>appropriate. So, to fill you in, I have road-tested Genomics Solution
>ProGest recently. It is specced at identifying 500fmol BSA in a gel slice.
>I confirm it can do that, but in my hands I have not got convincing data
>below that level, and I consistently get more peptides from a manual
>digest.
>
>So, new question: Is this sensitvity 'as low as one might expect' or can
>other vendors offer better specs. I would like to see data identifying at
>the 100fmol level. Is this realistic? Where are we at, on the evolution of
>digesters?
>
>Len
>
>
>
>
>
>
>>Proteomics is taking off with Genomics Solutions, Pharmacia, Micromass, PE
>>etc offering 'complete packges' anywhere from gel running through to ESItof
>>(some packages real, some due for 2000 launch). Central to the successful
>>operation of these setups must be an automated in-gel digestion system
>>whose ability to deliver on low abundance samples must match or exceed that
>>achievable through 'dirty' manual means.
>>
>>My question: who has experience of automated digesters and possibly
>>automated clean-up devices/spotters? Are they any good? Are they useful at
>>the sensitivity needed to identify Ag-stained spots?
>>
>>Any feedback welcome, especially on quantitation aspects
>
>*********************************************************************
>Dr Len C. Packman
>Assistant Director of Research
>Protein and Nucleic Acid Chemistry Facility
>Department of Biochemistry
>University of Cambridge
>80 Tennis Court Road
>Old Addenbrookes Site
>Cambridge, CB2 1GA, UK
>Tel: +44 (1223) 333639
>FAX: +44 (1223) 766002
>e-mail: lcp2@mole.bio.cam.ac.uk
>Visit my WWW page at http://www.bio.cam.ac.uk/proj/adr/PNAC/pnac.html
>
>
>
>
>
Bryan Dunbar
Protein Facility,
Room WT27,
Department of Molecular & Cell Biology,
University of Aberdeen,
Polwarth Building,
Foresterhill,
Aberdeen,
SCOTLAND.
U.K.
AB25 2ZD.
TEL: 01224-273103
FAX: 01224-273104
e-mail; b.dunbar@abdn.ac.uk