PepSyn
Krystyna Piotrowska (kpiotrow@interchange.ubc.ca)
Tue, 16 Nov 1999 15:12:55 -0800
Hello All,
We have attempted syntheses of two peptides 19-mer and 9-mer
with identical N-term. residues K-P-V-...
Both were synthesized with F-moc chemistry. 19-mer on 432A
9-mer 433A. Conductivity tracings for both of them do not
indicate any synthetic problems.
However, the first (19-mer) synthesis yielded almost 100%
HPLC pure product without N-terminal Lys.
The synthesis of the 9-mer gave 17% of the expected product,
the main product did not have Lys and there was 15% of peptide
without Lys and Pro.
It seems that Lys was coupled and then cleaved during deprotection
or workup.
Have anybody encounter similar problems. Any suggestions
how to solve the problem.
Thanks in advance,
Krystyna
Dr. Krystyna B.Piotrowska
Protein Service Laboratory, NAPS
Biotechnology Laboratory
Room 237, Wesbrook Building
6174 University Boulevard
University of British Columbia
Vancouver B.C., V6T 1Z3
Canada
Tel. (604) 822 9662, Fax (604) 822 0676
www.biotech.ubc.ca/naps/napshome. htm