Re: guanidine assay?

Dan Crimmins (crimmins@pathbox.wustl.edu)
Thu, 18 Nov 1999 12:41:28 -0600

At 09:40 AM 11/18/1999 -0500, Terry Sumpter wrote:
>Is anybody aware of a protocol for the quantitation of guanidine (to the
>low ppm level) from protein solutions ? Thanks.
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Terry,
I'm not sure what minimum level can be assayed, but SDS & GdmCl ppt quite
nicely and I have used High stock GdmCl solutions to estimate removal of
SDS from samples. The reverse should also work???

Regards,

Dan L. Crimmins
Washington University School of Medicine
Dept. Pathology/Division of Laboratory Medicine
660 S. Euclid Ave., Box 8118
St. Louis, MO 63110
Phone: 314-454-8514; Fax: 314-454-5208
e-mail: crimmins@labmed.wustl.edu