PepSyn

Thomas Andersen (AndersT@mail.amc.edu)
Fri, 19 Nov 1999 13:46:50 -0500

I need to synthesize a peptide and then couple it to a macromolecule, preferably through an amino group on my synthetic peptide. But I need to keep the N-terminus of the synthetic peptide free, as it is essential for activity. What is the best way to protect the a-amino group until I am ready to make it available?

a. Will keeping the FMOC on it work? Can I do that and couple through some distal Lys amino groups with N-hydroxysuccinimide activated macromolecule, and then deprotect the peptide?

b. Is there some other protection strategy that is good for this?

c.) Of course I can insert a Cys and couple through -SH, but I'd rather not if I don't need to.

d.) And I could get elaborate and engineer a site for proteolytic cleavage which would leave my desired N-terminus, but surely this won't be necessary?

I'd appreciate any thoughts.

Tom Andersen

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Thomas T. Andersen, Ph.D.
Protein Chemistry Core Facility
Albany Medical College
Albany, NY 12208
518 262-5368
518 262-5689 fax
anderst@mail.amc.edu