A possible source for labelled amino acids is Cambridge Isotopes.
There are basically 2 procedures for Fmoc acylation of amino acids:
using Fmoc chloride: J. Meienhofer et al. Int. J. Peptide Protein
Res. 13, 1979, 35-42
or by using Fmoc succinimide: G.F. Sigler, M. Goodman et. al.
Biopolymers, Vol 22 2157-2162
The Fmoc succinimide is the "milder" procedure. I prefer it. The key
issue is to precipitate the protonated Fmoc amino acid after the
acylation, ether and ethylacetate extractions. Most amino acids
precipitate (which means easy isolation and work-up) but very
hydrophilic (unusual) amino acids may not.
Then it may become difficult and tedious to isolate/purify the Fmoc
amino acid (I did it once by gelfiltration).
Good luck!
Henriette
Henriette A. Remmer, Ph.D
Director
Protein Sciences Facility
Biotechnology Center
University of Illinois
311/315 Noyes Laboratory, Box 62-1
505 S. Mathews Ave.
Urbana,IL 61801
Tel: (217) 333-4695 (Lab) (217) 333-3841 (office)
Fax: (217) 244-1142
homepage: http://www.life.uiuc/biotech/protein_sc.html
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