>Hi everyone,
> We have been seeing green smears up whole lanes on gels we have been
>running. It is project specific, and has just recently shown up. The
>chromatograms have sequence, but also overlaying the regular sequence are
>peaks of one color-all the way through. We are using anchor primers,
>sequencing 3' ends. Some runs have more green lanes than others, and it is
>completely random as to what machine, plates etc. Has anyone else ever seen
>anything like this, and if so-do you know what it is from ??
>Thanks,
>Jayne Lynch
>Senior Research Specialist
>Keck Center-UIUC