The purpose of this message is to announce the DNA Sequencing Research Group's
(DSRG) 2000 Study. In this two-part study we will focus on factors that affect
the accuracy and quality of sequence when Bacterial Artificial Chromosomes
(BACs) are used as the sequencing template. This study is being conducted with
a view towards establishing official DSRG recommended methods. Since the large
scale production of BACs is more problematic than that of standard plasmid
templates we will not be able to send the sample materials out in a mass
mailing as we have done in the past (e.g. Standard and Difficult Template
Studies). In order to participate in this study it will be necessary to request
materials in advance.
Part 1 of the study will concentrate on the operational steps directly related
to the sequencing of BACs. The DSRG will provide all registered participants
with a sample of a standard BAC and an appropriate primer. Participants will
then sequence the standard BAC using the primer provided and the methods
developed in their respective labs. We request that they send us the sample
file electronically, in either .scf or .abi format, and a completed web form
concerning the methodologies used to produce the sequence. The sequencing data
will be analyzed and compared with the information available on the web form
with a view towards identifying methods that correlate well with high quality
sequence. Preliminary results will be presented at ABRF 2000.
Part 2 of this study will concentrate on the operational steps relating to the
isolation of BAC templates for use in sequencing. The DSRG will provide all
registered participants with an agarose stab of bacteria containing the
standard BAC. Participants will then grow up the bacteria and isolate the BAC
by the methodologies utilized in their respective laboratories. We request that
they send the purified BAC to us for sequencing along with a completed web form
concerning the methods used in the isolation of this material. The sequencing
results will be analyzed and compared with the information available on the web
form in order to identify practices that correlate well with high quality
sequence. These will form the basis of the recommended method. Preliminary
results will be presented at ABRF 2000.
It is not be necessary to register for both parts in order to participate in
this study, although we encourage everybody to do so. To request the materials
necessary to participate in this study please use the following url:
http://brcweb.bio.cornell.edu/dsrg2000/
You will receive your sample of the standard BAC and/or the bacterial stab
shortly after registering. Additional information concerning how to return the
sequence, the purified BAC, where to find the web forms etc. will be provided
at that time.
Please note: In conjunction with the 2000 Study we are also conducting a
general survey of sequencing laboratories designed to give us an accurate
picture of the composition and configuration of the average DNA sequencing
laboratory. To take part in this survey please use the url given above.
I hope to hear from you all shortly!
Sincerely,
T. W. Thannhauser, Ph.D
Chair, DSRG
Theodore W. Thannhauser, Ph.D.
Director, BioResource Center
Rm. 149 Biotechnology Building
Cornell University
Ithaca, New York 14853-2703
Phone: (607) 254-4850
FAX: (607) 254-4847
Email: tt34@cornell.edu
http://brcweb.bio.cornell.edu
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The purpose of this message is to announce the DNA Sequencing Research
Group's (DSRG) 2000 Study. In this two-part study we will focus on
factors that affect the accuracy and quality of sequence when Bacterial
Artificial Chromosomes (BACs) are used as the sequencing template. This
study is being conducted with a view towards establishing official DSRG
recommended methods. Since the large scale production of BACs is more
problematic than that of standard plasmid templates we will not be able
to send the sample materials out in a mass mailing as we have done in the
past (e.g. Standard and Difficult Template Studies). In order to
participate in this study it will be necessary to request materials in
advance.
Part 1 of the study will concentrate on the operational steps directly
related to the sequencing of BACs. The DSRG will provide all registered
participants with a sample of a standard BAC and an appropriate
primer. Participants will then sequence the standard BAC using the
primer provided and the methods developed in their respective labs. We
request that they send us the sample file electronically, in either .scf
or .abi format, and a completed web form concerning the methodologies
used to produce the sequence. The sequencing data will be analyzed and
compared with the information available on the web form with a view
towards identifying methods that correlate well with high quality
sequence. Preliminary results will be presented at ABRF 2000.
Part 2 of this study will concentrate on the operational steps relating
to the isolation of BAC templates for use in sequencing. The DSRG will
provide all registered participants with an agarose stab of bacteria
containing the standard BAC. Participants will then grow up the bacteria
and isolate the BAC by the methodologies utilized in their respective
laboratories. We request that they send the purified BAC to us for
sequencing along with a completed web form concerning the methods used in
the isolation of this material. The sequencing results will be analyzed
and compared with the information available on the web form in order to
identify practices that correlate well with high quality sequence. These
will form the basis of the recommended method. Preliminary results will
be presented at ABRF 2000.
It is not be necessary to register for both parts in order to participate
in this study, although we encourage everybody to do so. To request the
materials necessary to participate in this study please use the following
url:
http://brcweb.bio.cornell.edu/dsrg2000/
You will receive your sample of the standard BAC and/or
the bacterial stab shortly after registering. Additional information
concerning how to return the sequence, the purified BAC, where to find
the web forms etc. will be provided at that time.
Please note: In conjunction with the 2000 Study we are also conducting a
general survey of sequencing laboratories designed to give us an accurate
picture of the composition and configuration of the average DNA
sequencing laboratory. To take part in this survey please use the url
given above.
I hope to hear from you all shortly!
Sincerely,
T. W. Thannhauser, Ph.D
Chair, DSRG
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