Has anyone has experience with CY3 or CY5 labelling ? Pharmacia recommends
to use very mild condition for deprotection. I understand perfectly that PAC
chemistry would be the best but I cannot use it at the moment Do you think
I can use normal protecting groups (like CBz, Abz and Gibu) anyway.? Does
24h at RT or 16h 37C should be better than 6h 55C ?
What about the amidite concentration used ? Is 100 mM not too high ? What
about the coupling time and the coupling yield ?
On the Cy3 and Cy5 there is a MMT protecting on the primary alcool . From
my experience the MMT protecting group is normaly less labile than DMT on
the MMT-NH-linker for example and a special procedure for deprotection is
necessary in this case . Should I use a special procedure for the last
de-MMTrytilation on CY3 and Cy5 ?
Thank you very much for any ideas
Best regards
Alain
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Alain LAURENT Ph. D
Oligonucleotide Chemistry
ESGS Groupe CYBERGENE
11, rue Claude Bernard
35 400 Saint MAL0
FRANCE
Tel: +33 (0)2 99 21 90 40
Fax: +33 (0)2 99 21 90 41
e-mail: a.laurent@eurosequence.com
http://www.eurosequence.com
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