Re: AAA - yeast cell lysate

Laurey Steinke (lsteinke@molbio.unmc.edu)
Mon, 29 Nov 1999 14:55:22 -0600

Messages sorted by: [ date ][ thread ][ subject ][ author ]
Next message: Joan Oefner: "Analysis of Well Characterized Biotechnology Pharmaceuticals,"
Previous message: Amina S. Woods: "RE: mass spec: maldi of phage coat protein"
Maybe in reply to: Angela c. Murphy: "AAA - yeast cell lysate"
Next in thread: Chris Wood: "Re: AAA - yeast cell lysate"

Hello Angela,
I haven't seen any replies to you, so I will put in my two cents worth. I
have quantitated free leucine from rat liver extracts on my Beckman 6300.
The investigator uses a salicylic acid precipitation of the proteins (it
worked better than perchloric precipitation). I took several aliquots of
the supernatent (different amounts), dried them down, and resuspended in
Na-S. The proceedure worked well and reproducibly. The results for the
controls agreed with literature values. If your investigator would like
the exact proceedure, I can dig it up.

Good Luck!!
-laurey

Weather Inconsequential: A beautiful crisp sunny day. The leaves are off
of all the trees I can see from my window. Hope you all had a great
Thanksgiving!
Laurey Steinke
Protein Structure Core Facility
University of Nebraska Medical Center
Omaha Nebraska, 68198-4525

Phone (402) 559-6647
FAX (402) 559-6650
lsteinke@molbio.unmc.edu

Next message: Joan Oefner: "Analysis of Well Characterized Biotechnology Pharmaceuticals,"
Previous message: Amina S. Woods: "RE: mass spec: maldi of phage coat protein"
Maybe in reply to: Angela c. Murphy: "AAA - yeast cell lysate"
Next in thread: Chris Wood: "Re: AAA - yeast cell lysate"