RE: Dissolving PLP 9-mer peptides
John.Stewart@UCHSC.edu
Tue, 30 Nov 1999 17:31:55 -0700
Two possible methods:
1. Dissolve the peptide in a minimum amount of pure DMSO, or
DMSO:water (1:1). Dilute to working concentrations with water (water is a
better solvent than PBS, due to the salting out effect of PBS). If your
peptides remain in solution in an acceptable concentration of DMSO, this is
a siimple method.
2. A. Dissolve the peptide in glacial acetic acid, 1 mg/mL.
B. Dissolve dextran (clinical grade, MW 60,000-90,000) in water,
20mg/mL.
C. Prepare small glass vials (acid-washed, rinsed, dried).
D. To each vial add: 100 microliters peptide solution, 100
microliters of dextran solution and 300 microliters of water. Lyophilize.
Store at -20 degrees.
E. Reconstitute by adding 100 microliters of PBS to a vial. The
resulting solution is 1.0 microgram peptide per microliter of solution,
containing 2% dextran.
Even Method 2 may not work for your peptides, but it is worth a try.
John M. Stewart
Dept. of Biochemistry B126
Univ. of Colorado Medical School
Denver, CO 80262
Phone: 303-315-7534
FAX: 303-315-8215
Email: john.stewart@uchsc.edu