AHA! If it is brand-spanking-new, have you previously
injected any peptide/protein on it, and how much peptide did you
inject? Have never seen a chromatography support that doesn't "eat"
some of the first sample put through it! If this was a large shot of
peptide, what I said is not relevant.
>iii) The column I'm using is a brand-spanking-new Vydac VHP (DEAE) 5.00mm
>ID column. I'm told these shouldn't really have any metal atoms lying
>around, because of the type of matrix (spherical PS-DVB beads). I doubt
>it'd be bad enough to chelate everything I threw at it, anyway(?)
David
David A. Schooley
Dept. of Biochemistry/330
Univ. of Nevada
Reno, NV 89557
schooley@unr.edu
tel: (775) 784-4136; fax (775) 784-1419
NOTE NEW AREA CODE: Mandatory after 5/15/99