So far we aren't having much luck, lots of streaking (despite delipidation),
extremely poor reproducibility basically due to the pretty poor solubility
of the proteins in the common non-ionic 2D detergents we've tried.
At a meeting i was at recently, one of the speakers mentioned (in passing) a
technique that i think he called zone (zonal?) electrophresis and put up a
2D like gel. The inference was that if you couldn't get the hydrophobic
proteins you were interested in to run on standard 2D you could use this
weaker resolution technique which had the advantage of being able to use SDS
in both dimensions. The only zone electrophoresis i've ever heard of is CZE,
and this certainly wasn't it. I may have misheard, but in that case so did
the guy sitting next to me as we both agreed on what was said. Anyone out
there know what I am babbling about?
Alternatively - anyone got any suggestions for some good, novel detergents
to try and where to get them?
thanks for the advice,
Derek Ellison
Medeva Development
Speke
Liverpool
L24 9GR
Phone: 44 151 705 5442
Fax 44 151 705 5189
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