Alpert was probably suggesting pH 2 for strong cation exchange, not anion
exchange. At that pH your carboxyls will be protonated and thus not
exchangeable. Lower the pH to 5.5 and try it with the 10% MeCN . Wait for
10 column volumes of solvent to equilibrate it. You might consider a
wavelength of 214 instead.
Please let us know how you are doing. This is very instructive
Amos
Amos Heckendorf (nestgrp@world.std.com)
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