Re: DNA SEQ: Straight lines tracking when using paper combs
Derek Harkins (derek_harkins@ncsu.edu)
Sun, 05 Dec 1999 18:41:28 -0500
Tony Tarragona wrote:
>
> Dear Sequencers,
>
> Since we have been using the 96 lane paper combs, the tracking is gone
> really bad.
> We follow the procedure from ABI, pre-run, pause, load odds, start run, 2
> min, pause, load evens, run for 2 min, pause, take comb out, run.
> Since using the paper combs we have notice that before the dyes appear we
> have a flurry of horizontal fluorescent lines, would that be the cause for
> the tracking to fail?
> ABI does not know, they tell me to upload the gel at their website, but the
> access is denied even with a username and password that they provide.
>
> I wonder if any of you has seen this and how have you solve it.
>
> Many thanks
>
> tony
Hello all
When you say paper combs, do you mean the sharkstooth paper combs that
swell once they are immersed in the buffer, or are you referring to
membrane combs?
Cheers
Derek
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.-. .-. .-.Derek M. Harkins <derek_harkins@ncsu.edu>.-. .-. .-.
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