Re: biogenic amines

David A. Schooley (schooley@med.unr.edu)
Tue, 7 Dec 1999 11:30:17 -0800

Hong-
Catecholamines are so readily degraded that many advocate
sacrificing rats with microwave to denature the degradative enzymes-
levels of biogenic amines in microwave-sacrificed rats are
significantly higher than rats conventional sacricied and then
dissected and extracted. See references:

Mir A. K., Pallot D. J. and Nahorski S. R. (1983) Biogenic
amine-stimulated cyclic adenosine-3',5'-monophosphate formation in
the rat carotid body. J. Neurochem. 41, 663-669

Moroji T., Takahashi K., Ogura K., Toishi T. and Arai S. (1977) Rapid
microwave fixation of rat brain. J. Microw. Power 12, 273-86,

>Are there any classic protocols for the isolation and purification of
>biogenic amines from tisseus? I am aware that it's very important to prevent
>the oxidation of such compounds. What are the best anioxidants or inhibitors
>for my purpose? Thanks for your feedback.
>
>Hong Li

David

David A. Schooley
Dept. of Biochemistry/330
Univ. of Nevada
Reno, NV 89557
schooley@unr.edu
tel: (775) 784-4136; fax (775) 784-1419
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