What's the best combination of parameters (voltages and capillary temp) to
promote formation of doubly charged ions in nanospray MS? I get quite a few
singly charged ions in the 1000-2000 m/z range whose 2+ states can be found
on zoomscan but which are not obvious by inspection of the full scan. What
tricks favour the higher charge state? I'm spraying in 1% formic acid, 70%
MeOH.
Len
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Dr Len C. Packman
Assistant Director of Research
Protein and Nucleic Acid Chemistry Facility
Department of Biochemistry
University of Cambridge
80 Tennis Court Road
Old Addenbrookes Site
Cambridge, CB2 1GA, UK
Tel: +44 (1223) 333639
FAX: +44 (1223) 766002
e-mail: lcp2@mole.bio.cam.ac.uk
Visit my WWW page at http://www.bio.cam.ac.uk/proj/adr/PNAC/pnac.html