Light scattering detectors

VERNON SHOUP (vernon.shoup@regpha.com)
08 Dec 99 09:36:45 +0000

We are considering buying an economical light scattering detector for HPLC, for characterizing protein multimers/aggregates and PEGylated proteins.
Wyatt's low-end instrument is the triple-angle (45, 90, 135 degrees) MiniDawn. Precision Detectors' low-end instrument is the single-angle (90 degrees) PD2010. More advanced instruments use more angles and/or QELS.

I'd appreciate some feedback from light-scattering detector users. In particular, for my purposes, does one need multiple-angle detection, or is just a scattering angle of 90 degrees sufficient? How important is temperature control? How easy to use are the various manufacturers' software packages?

Finally, can someone tell me what QELS (Dynamic Scattering) is?

Vernon

Vernon A. Shoup
Regeneron Pharmaceuticals, QC Dept.
Rensselaer, NY 12144

vernon.shoup@regpha.com