Re: MassSpec: LCQ

Jose Cesar Rosa (jrosa@cisunix.unh.edu)
Thu, 09 Dec 1999 10:01:13 -0800

>Len,
I am a novice on this area, but maybe you can use Bradykinin, the 2+ state
and 1+ state are very dependable of concentration and use this one for
tunning LCQ, you can also spike your sample with this peptide to find the
optimum concentration for 2+ stage.
Cesar

>>A question for experienced LCQ users:
>>
>>What's the best combination of parameters (voltages and capillary temp) to
>>promote formation of doubly charged ions in nanospray MS? I get quite a few
>>singly charged ions in the 1000-2000 m/z range whose 2+ states can be found
>>on zoomscan but which are not obvious by inspection of the full scan. What
>>tricks favour the higher charge state? I'm spraying in 1% formic acid, 70%
>>MeOH.
>>
>>Len
>>
>>*********************************************************************
>>Dr Len C. Packman
>>Assistant Director of Research
>>Protein and Nucleic Acid Chemistry Facility
>>Department of Biochemistry
>>University of Cambridge
>>80 Tennis Court Road
>>Old Addenbrookes Site
>>Cambridge, CB2 1GA, UK
>>Tel: +44 (1223) 333639
>>FAX: +44 (1223) 766002
>>e-mail: lcp2@mole.bio.cam.ac.uk
>>Visit my WWW page at http://www.bio.cam.ac.uk/proj/adr/PNAC/pnac.html
>>
>>
>>
>>
>
Jose Cesar Rosa PhD
Dept. of Chemistry
University of New Hampshire
23 College rd. Parsons Hall
Durham NH 03824
phone: (603) 862-3797