Re: MassSpec: LCQ

Jose Cesar Rosa (jrosa@cisunix.unh.edu)
Thu, 09 Dec 1999 10:06:39 -0800

Len,
I am a novice on this area, but maybe you can use Bradykinin, the 2+ state
and 1+ state are very dependable of concentration and use this one for
tunning LCQ, you can also spike your sample with this peptide to find the
optimum concentration for 2+ stage.
Cesar

>>A question for experienced LCQ users:
>>
>>What's the best combination of parameters (voltages and capillary temp) to
>>promote formation of doubly charged ions in nanospray MS? I get quite a few
>>singly charged ions in the 1000-2000 m/z range whose 2+ states can be found
>>on zoomscan but which are not obvious by inspection of the full scan. What
>>tricks favour the higher charge state? I'm spraying in 1% formic acid, 70%
>>MeOH.
>>
>>Len

Jose Cesar Rosa PhD
Dept. of Chemistry
University of New Hampshire
23 College rd. Parsons Hall
Durham NH 03824
phone: (603) 862-3797