We are thinking of trying a commercial 100 micron column (or smaller, if
available) for LC/MS on an LCQ, and I was wondering what experience the
ABRF people have with the commercial versions of these columns. What is
maximum flow they can tolerate and what is normal operating flow that you
use them at, and are there any tricks you've found helpful?
We've been using peptide traps, but I understand there are now membranes
available for lower amounts of material. I would also be interested in
experiences of the group with these membranes, and which ones have proven
most useful.
Katheryn Resing