Sequence Analysis and G4's

From: Robert Lyons (boblyons@umich.edu)
Date: Fri Dec 17 1999 - 15:01:18 EST


I recently received several new Mac G4 systems, part of a push
by UMich to retire older Macs by giving us new ones very cheap.
Nice deal. Problem is, Sequence Analysis V3.3 won't run on the
new machines (running under MacOS9).

Using a good gel file, I can start up SeqAnal and ask it to
'Track and Extract' the gel. Seems to work fine through the re-
tracking process (*very* fast!), then when the Sample Manager
window pops up to start the basecalling routine, it stalls. No
progress apparent on the first lane, and do just about any-
thing (click, scroll, etc), the entire system locks up.

Two different gel files do the same thing. I've re-installed Seq
Anal program multiple times. Memory is allocated well above that
requested by the SeqAnal and basecaller programs. Virtual Mem
(a common culprit in Mac freezes) can be on or off, with no
change in behavior. I've not tried running under MacOS8.6 yet.

PE Biosystems Tech Support was contacted, and their response
wasn't very satisfactory. They said "we haven't tested the
program on that configuration, and do not support it".
I would have felt better if they had at least said they were
working on it.

So - has anyone else tried to run Sequence Analysis 3.3 on a
G4/MacOS9 system? any hints?

Bob Lyons
University of Michigan



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