Dear Chris:
Other than by pH <3, the only way I know of disrupting this interaction is
said to be by sulfhydryl reduction with MSH. This was reported in
Biotechniques, about 10 issues back. It may matter whether your
avidin/strepavidin has been cross-linked. I have no experience with these
procedures. Your bait may not survive these procedures either. There is a
monomeric strepavidin of lower affinity. I hope someone else can share
his/her experience with that reagent.
Tony
At 02:55 PM 12/17/99 -0800, you wrote:
>
> I would like to find out how one can disrupt the
>avidin/streptavidin-biotin complex. I want to exploit this high affinity
>interaction for protein purification. At the end I would like to elute the
>biotinylated bait from the avidin/streptavidin resin and don't know how to
>go about it.
>
> Thanks,
>
>Chris (turck@itsa.ucsf.edu)
>
************************************
Anthony T. Yeung, Ph.D.
Director, Fannie E. Rippel Biotechnology Facility
Member, Institute for Cancer Research
Fox Chase Cancer Center
7701 Burholme Ave., Philadelphia, PA 19111
Voice: 215-728-2488 FAX: 215-728-3647 email: AT_Yeung@FCCC.edu
Public Key: 4F3A 98D3 AC8B B56D A49B 4CD6 3057 376A 2951 68A4
http://www.fccc.edu/research/labs/yeung/
************************************
This archive was generated by hypermail 2b29 : Wed Jan 12 2000 - 16:13:53 EST