Jean,
I don't know if there are commercially available thio-ester resins, but I am
sure that you will find the following references of help either in making the
resins or using the subsequent products:
James Blake and Choh Hao Li, Proc.Natl.Acad.Sci. USA, 78, 4055-4058. "New
segment-coupling method for peptide synthesis in aqueous solution: Application
to synthesis of human [Gly17]-beta-endorphin"
James Blake, Int.J.PeptideProteinRes., 17, 1981, 273-274. "Peptide Segment
Coupling in Aqueous Medium: Silver Ion Activation of the Thiolcarboxyl Group"
Donald Yamashiro and James Blake, Int.J.PeptideProteinRes., 18, 1981, 383-392.
"Use of Thiol Acids in Peptide Segment Coupling in Non-Aqueous Solvents"
James Blake, Int.J.PeptideProteinRes., 27, 1986 191-200. "Total synthesis of
S-carbamoylmethyl bovine apocytochrome c by segment coupling"
James Blake, Donald Yamashiro, K. Ramasharma and Choh Hao Li,
Int.J.PeptideProteinRes., 28, 1986, 468-476. "Chemical synthesis of
alpha-inhibin-92 by the thiocarboxyl segment coupling method"
Donald Yamashiro and Choh Hao Li, Int.J.PeptideProteinRes., 31, 1988, 322-334.
"New segment synthesis of alpha-inhibin-92 by the acyl disulfide method"
Best regards,
Richard Noble
PE Biosystems
email: noblern@pebio.com
PS. I remember Jim telling me that his coworkers did not appreciate his use of
H2S.....wonder why???? Obviously it led to less obnoxious methods of generating
the thio-containing materials.
---------------------- Forwarded by Richard Noble/FOS/PEC on 12/20/99 03:16 PM
---------------------------
"Lefebvre, Jean" <Jean.Lefebvre@nrc.ca> on 12/20/99 10:51:43 AM
To: Recipients of ABRF List <abrf@aecom.yu.edu>
cc: (bcc: Richard Noble/FOS/PEC)
Subject: Peptide Synthesis: Thioester Resin
> Dear ABRF members,
>
> do you know if generating thio-ester resin is availlable for Fmoc
> Chemistry.
>
> Season Greetings!
>
> ===========================================================
> Jean Lefebvre
> Biotechnology Research Institute
> National Research Council of Canada
> 6100 Royalmount Avenue
> Montreal, QC
> Canada, H4P 2R2
>
> Phone: (514) 496-6167
> Fax: (514) 496-5143
> E-mail: jean.lefebvre@nrc.ca
> ===========================================================
>
>
>
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