Reply to: N-terminal sequencer's
Hello All and Merry Christmas,
Well merry for some of us. I'm in a delemia. Just recently my Procise Seq is only producing 10% yields on B-lact standard run. I'm not sure why this is occuring. There is also an ugly, extreemly large peak, which I've been told is analine, in the first residue cycle. This peak then drops off in further residue cycles. To reduce the aniline peak, I've done various washes with 70% ACN 0.1% TFA, MQ, MeOH and finally S2B by using the cleanup menu. But I think the aniline and reduced yields are seperate issues. >From what I've worked out, it can't be in the coupling or the cleavage stages b/c otherwise there would be carryover. This we are not seeing. It can't be in the conversion b/c otherwise we'd see shadow peaks which we aren't seeing.
Its not the column b/c the standards are fine and it must be in the flask or chemistry b/c the blank is ok too. I recently changed the flask, optimised the post/pre conversion times and changed R2B to try and find a solution. Anyone out there before the christmas period that can help with any suggestions would be much appreciated. Thanks in advance
Janice Duff
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