cyclization to pyroglutamate

From: Danny Kitsberg (kitsberg@alomone.com)
Date: Wed Dec 29 1999 - 08:20:42 EST


I have a peptide that has Gln at its C-terminus that I am trying to
cyclize to pyroglutamate. The conditions that I standardly use for this
reaction is 5% (v/v) acetic acid at 65 deg for 3.5hrs and this normally
works well. However, I now have a peptide that is sensitive to acidic
conditions and I was wondering whether anyone had suggestions for more
mild (or at least less acidic) conditions for cyclization.
Thank you
Danny Kitsberg

-- 
Danny Kitsberg Ph.D.
Alomone Labs
POB 4287
Jerusalem
91042
Israel

Tel: 972-2-587-1102 FAX: 972-2-587-1101



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