I have a peptide that has Gln at its C-terminus that I am trying to
cyclize to pyroglutamate. The conditions that I standardly use for this
reaction is 5% (v/v) acetic acid at 65 deg for 3.5hrs and this normally
works well. However, I now have a peptide that is sensitive to acidic
conditions and I was wondering whether anyone had suggestions for more
mild (or at least less acidic) conditions for cyclization.
Thank you
Danny Kitsberg
-- Danny Kitsberg Ph.D. Alomone Labs POB 4287 Jerusalem 91042 IsraelTel: 972-2-587-1102 FAX: 972-2-587-1101
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