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Journal of Biomolecular TechniquesDNA from Buccal Swabs Suitable for High-Throughput SNP Multiplex AnalysisWe sought a convenient and reliable method for collection of genetic material that is inexpensive and noninvasive and suitable for self-collection and mailing and a compatible, commercial DNA extraction protocol to meet quantitative and qualitative requirements for high-throughput single nucleotide polymorphism (SNP) multiplex analysis on an automated platform. Buccal swabs were collected from 34 individuals as part of a pilot study to test commercially available buccal swabs and DNA extraction kits. ....Read more A Pair of Ligation-Independent Escherichia coli Expression Vectors for Rapid Addition of a Polyhistidine Affinity Tag to the N- or C-Termini of Recombinant Proteins6×His tag is one of the most widely used affinity fusion tags that facilitates detection and purification of recombinant proteins. However, the location of this tag within a particular type of protein may influence the expression, solubility, and bioactivity of the protein, and the optimal location needs to be determined experimentally. To provide a tool for rapid generation of 6× His tags at the N- or C-terminus of any recombinant protein, we have constructed a pair of Escherichia coli expression vectors—pLIC-NHis and pLIC-CHis—based on the pET30a vector, for ligation-independent cloning (LIC)...Read more Isolation of Bothrops jararaca Snake Antithrombin from the Supernatant of Fibrinogen PurificationA novel method of antithrombin (AT) purification from Bothrops jararaca snake plasma was developed to obtain this protein using a waste supernatant from B. jararaca fibrinogen purification. The AT purification was achieved by affinity chromatography on HiTrap Heparin HP. The results showed an efficient purification process yielding pure AT (purity 65-fold and specific activity 368.91)... Read more ABRF Discussion ForumMar 19, 2010, by suzanne perrySubject: RE: Autosampler Problems with Waters nanoACQUITY attached to an Orbitrap Mass Spec Mar 19, 2010, by Steve HartsonABRF NewsFinance Committee needs new membersDo you have some experience in
investments/stock market? ABRF2010: Pre-Meeting Tours of Core Laboratories at UC DavisThe CTSA Leadership at UC Davis has graciously agreed to conduct tours of some of their core resources associated with the support of the Clinical and Translational Science Center. ABRF 2010: "Translating Basic Research With Advances in Biomolecular Technology"Registration for the ABRF2010 meeting is now open! National Science Foundation Educational Workshop Scholarships!The NSF, in partnership with the ABRF and Nemours, is sponsoring 25 Satellite Workshop Awards at ABRF2010 to promote participation in this acclaimed series of technology-focused workshops. Deadline for applications HAS BEEN EXTENDED TO MARCH 1, 2010! ABRF/FASEB Career Fair and Employment Service at ABRF2010There will be a Job Fair and Employment Service available to attendees of this year’s meeting in Sacramento.This is a FREE service for job-seekers. Click "Read More" for more details! Dr. Patrick Brown, Stanford University, has been chosen as this year's ABRF Award RecipientPatrick Brown has been selected as the 2010 ABRF Award recipient, in recognition of his pioneering work in the development of microarrays, and the diverse applications of this technology in genetic research. Five exciting pre-meeting Educational Workshops announced for ABRF 2010 * Proteomics Platforms, * Targeted Proteomics, * RNA ABCs, * NextGen DNA Sequencing Analysis, and * Lab Management Skills.
PRG 2010 Study now open!The Proteomics Research Group invites you to participate in a study that explores the use of different approaches for determining sample composition and identifying unforeseen problems in otherwise ‘straight-forward’ proteomics analyses. 2010 NCRR announcement for Shared Instrumentation GrantsPAR-10-082 (2010 SIG awards announcement) is now posted. A new maximum award of $600,000 has been set, and approximately 125 awards will be funded. Deadline for applications is 3/23/10. Upcoming Meetings of InterestJul. 12-14, 20102010 International Conference on Bioinformatics, Computational Biology, Genomics and Chemoinformatics (BCBGC-10)the Imperial Swan Hotel & Suites, 7050 South Kirkman Road, Orlando, Florida, United States 24th Annual Symposium of The Protein SocietyLooking at Proteins: Expanding Perspectives and New Technologies Manchester Grand Hyatt, San Diego, CA, United States Complex Carbohydrate Research Center coursesCCRC , The University of Georgia, Athans, Georgia, United States |
