| Protein Expression Research Group (PERG) |
| |
Current Members |
Studies |
Activities |
Membership History |
|
 |
| The mission of the Protein Expression Research Group (PERG) is (1) to educate ABRF members and scientific community on best practices in recombinant protein expression and purification, and (2) help to advance the technology in recombinant protein expression and purification as a public service for laboratories engaged in protein expression and purification.
Scientific areas include eukaryotic expression (baculovirus/insect cells, and mammalian), bacterial expression, cell-free expression, construct design, protein purification (tags, increasing throughput, increasing ease), protein refolding, and cell culture. |
|
| Current Membership |
Dr Fei P. Gao
(Chair) - University of Kansas
Pamela Scott Adams
- Trudeau Institute
Dr. James W. Bryson
- Bristol-Myers Squibb Co.
Dr Richard J Heath
- St Jude Children's Research Hospital
Dr. Cynthia Kinsland
- Cornell University
Dr Thomas Neubert
(EB liaison) - New York Univ Sch of Med
|
|
| Studies |
| 1) |
Recombinant Protein Expression and Purification Study (Completed): Participants in the study were sent a plasmid containing the gene for yeast ADH. Study participants expressed this protein in bacteria and purified it using metal chelation affinity chromatography. From the data returned, it appears as if protein labs do not use a standardized protocol, however, for this protein at least, all methods of expression and purification gave active enzyme. Results were presented at ABRF 2009 in Memphis, TN.
- PERG ADH Expression Study
(542K)
|
| 2) |
PERG Survey 2007 (Completed). Slides are available summarizing the survey conducted by the ABRF Protein Expression Research Group, February-March 2007. These slides were presented at the Protein Expression Research Group presentation at ABRF 2007 at the Tampa Convention Center, Tampa, Florida.
- PERG_Survey2007_Slides
(18K)
|
|
|
| Activities |
| 1) |
Recombinant Protein Laboratory Workshop held as a Satellite to the ABRF 2009 meeting in Memphis. This two day educational workshop provided an opportunity for participants to purify proteins from cell pellets using a variety of resins and perform several analyses to assess purity and yield. Both conventional procedures and an automated system were used. Participants also used a novel high-throughput screening approach to assess solubility conditions. Basic theory was covered during interactive discussion sessions.
- Solubility Scoring Sheet
- Protocol Booklet
(157K)
- Solubility Screening
(155K)
- Basics of Protein Expression
(1,875K)
- Affinity Purification
(8,160K)
|
| 2) |
State of the art technologies for producing high-quality recombinant proteins in core facilities
- Flyer
(171K)
|
| 3) |
David Wasilko: "Titerless infected insect cell preservation and scale-up (TIPS)"
- David Wasilko
(1,028K)
|
| 4) |
Francis Rajamohan: "Expression, optimization and production of recombinant proteins in insect cells using baculovirus"
- Francis Rajamohan
(3,514K)
|
| 5) |
Frederic Boyce: "Baculovirus-mediated gene transfer into mammalian cells"
- Frederic Boyce
(2,763K)
|
| 6) |
Jim Bryson: "Design of protein constructs for optimal expression and efficient methods for generating DNA expression vectors
- Jim Bryson
(5,187K)
|
| 7) |
John Hawes: "Characterization of recombinant protein reagent to assure high quality"
- John Hawes
(454K)
|
| 8) |
Rebecca Page: "Optimization and increasing throughput of expression in E. coli"
- Rebecca Page
(9,457K)
|
| 9) |
Yaeto Endo: "Cell-Free recombinant protein expression"
- Yaeto Endo
(17,691K)
|
| 10) |
PERG Survey: "Bottlenecks in protein expression" 2007
- PERG Survey
(102K)
|
|
|
| Membership History |
| Member Name |
Organization |
Details |
|
Pamela Scott Adams |
Trudeau Institute |
Ad hocEB liaison:
02/09 -
03/10
|
|
Michael Doyle |
Bristol-Myers Squibb Pharma |
EB Liaison:
05/06 -
02/08
|
|
Dr. Arnold M. Falick |
HHMI-UC Berkeley |
EB liaison:
02/08 -
02/09
|
|
Dr Fei P. Gao |
University of Kansas |
Member:
03/11 -
02/12
|
|
Dr. John W. Hawes |
Miami University |
Chair:
02/08 -
03/09
Member:
05/06 -
02/08
Member:
03/09 -
12/10
|
|
Dr Richard J Heath |
St Jude Children's Research Hospital |
Chair:
03/09 -
05/10
Member:
03/08 -
03/09
|
|
Dr. Cynthia Kinsland |
Cornell University |
Chair:
05/10 -
02/12
Member:
09/07 -
05/10
|
|
Rebecca Page |
Brown University |
Member:
06/07 -
02/11
|
|
Francis Rajamohan |
Pfizer Inc. |
Chair:
10/06 -
02/08
Member:
02/08 -
03/09
Member:
05/06 -
10/06
|
|
Chris W Turck |
Max Planck Institute of Psychiatry |
EB liaison:
04/10 -
02/11
|
|
