Date: Fri, 9 May 2008 14:53:44 -0400 From: "Matthias Trost" <matthias.trost@gmail.com> To: "ABRF Discussion List" <ABRF@list.abrf.org> Subject: multistage activation on LTQ-OrbitrapHi, I would like to try some multistage activation ("Pseude-MS3") on phospho-peptides on our Orbitrap. I have found where to click the bottom "multistage activation", but then it asks for neutral mass losses (still easy: -98,-49 etc). But when it asks for Product ion masses, I am lost ... Would anyone be so kind and share his/her knowledge in telling an Orbitrap how to activate multistage activation? I cannot get anyone from Thermo on the line (friday afternoon, nice weather). and I really would liek to do the testing this weekend. Thanks a lot, Matthias |