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RE: 3730 capillary regeneration
< Last Message by Thread: RE: 3730 capillary regeneration from Ashwani Kumar
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Date: Wed, 23 May 2007 08:57:32 -0700
From: "Chang Shi" <cshi@mclab.com>
To: "'ABRF Discussion List'" <ABRF@list.abrf.org>
Subject: RE: 3730 capillary regeneration

HI, Ashwani,

MCLAB has a product (CARE solution) for regenerating capillary Array
(including 50 cm), which can be used on the sequencer machine.  Any abrf
member can send a email (mclab@mclab.com) to get free bottles of the
solution  (the offer ends 6/30/07).

1).No need to remove the capillary for treatment
2).the resolution loss can be recovered after the treatment
3).To remove the background noise, e.g. yellow haze, and keep the baseline
low
4).It is easy to use.


Whenever there is dirty background or resolution loss, we use it to clean
the gel block and capillary.  It has been two years now.  The highest number
of runs is 3074 (it is still on the 3730xl, has not reach Glen Byrns's 9000
record).  

Hope it helps.

Changping Shi,  PH.D.
MCLAB
384 Oyster Point Blvd, Suit 15
South San Francisco,  CA 94080
Tel: (650)872-0245
Fax:(650)872-0253

-----Original Message-----
From: Ashwani Kumar
Sent: Tuesday, May 22, 2007 8:47 PM
To: 'ABRF Discussion List'
Subject: RE: 3730 capillary regeneration

Did anyone had any luck with 50cm array regeneration for sequencing on ABI
3730? We on an average use 50 cm arrays for 1500 runs, after which many of
the capillaries start showing resolution loss of the peaks in many of the
capillaries.


Ashwani
In-Charge, Sequencing Facility,
TCGA

-----Original Message-----
From: grbyrns@ucdavis.edu
Sent: 23 May 2007 02:05
To: ABRF@list.abrf.org
Subject: 3730 capillary regeneration

Just a quick update to those using the AB3730 sequencer.  We now get an
average of about 5000 runs per 36cm array.  Our highest is over 9000 runs.
Most for fragment analysis, maybe 5% sequencing.  
Our wash technique is to use the syringe to empty the array of polymer while
it stands on a paper towel.  Then, 1M Nitric acid is syringed into the array
and allowed to stand for 5 minutes.  It is then replaced with 1N NaOH, left
for 5 minutes.  Finally, at least 10 syringe-loads of hot ddH2O is washed
through the array before remounting.  The bubble wizard is used to fill the
array, followed by the spatial (with fill) calibration.  The two fills give
time for the polymer re-coating of the stripped capillaries.
Again, DO NOT TRY THIS, if you don't have a good way to move the liquids
under high pressure into and out of the array.  An exploding syringe of
nitric acid will ruin your day.

Glen Byrns
Veterinary Genetics Laboratory
UC Davis

 
Thread Index    [ Read All ] ^ Top
  Subject From  Received On  (local time) 
3730 capillary regeneration 
grbyrns@ucdavis.edu  22-May-07 16:35 (GMT -4) 
RE: 3730 capillary regeneration 
Ashwani Kumar  22-May-07 23:36 (GMT -4) 
You are here RE: 3730 capillary regeneration 
Chang Shi  23-May-07 11:57 (GMT -4)