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Submission Age Keyword(s)
Problem Category Instrument Model
Template Type Reaction Volume (µl)
Sequence Chemistry Dye Volume (µl)
Dilution Buffer Dilution Buffer Volume (µl)
Dilution Buffer Mfg
Additive Additive Concentration (mM)
Additive Type
Additive Mfg
Reaction Clean-up Resuspension/Loading in
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 Showing 1 - 30 of 132   
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View ID Current Primary Sort Direction Indicator. Click to reverse sort direction.Submit. Date Updated on Title Category Instrument Template Type Seq. Chem.
Click here to open a detailed view of this record 194 07-May-12 09-May-12 5x Sequencing Buffer 3.0 vs. 3.1 ! Please Help ! Applied Biosystems 3130/3130xl PCR product BigDye v3.1 or 4:1 BigDye v3.1:dGTP v3.0
Click here to open a detailed view of this record 193 21-Apr-12 11-May-12 Injection Stutter Sample Problem Applied Biosystems 3730/3730xl plasmid BigDye v3.1
Click here to open a detailed view of this record 192 11-Apr-12 09-May-12 Sequencing ! Please Help ! Applied Biosystems 3730/3730xl plasmid BigDye v3.1
Click here to open a detailed view of this record 191 09-Apr-12 09-May-12 PCR product sequencing ! Please Help ! Applied Biosystems 3730/3730xl PCR product BigDye v3.1
Click here to open a detailed view of this record 190 20-Mar-12 11-May-12 Abi3130xl, sample isn't injected Instrument Problem Applied Biosystems 3130/3130xl PCR product BigDye v3.1
Click here to open a detailed view of this record 189 12-Mar-12 21-Mar-12 alternative reagents Other Applied Biosystems 3130/3130xl PCR product BigDye v3.1
Click here to open a detailed view of this record 188 18-Jan-12 18-Jan-12 Baseline is high and colors are separated Instrument Problem Applied Biosystems 310 PCR product BigDye v1.1
Click here to open a detailed view of this record 187 28-Dec-11 01-Jan-12 3730xl Noise estimation failure Instrument Problem Applied Biosystems 3730/3730xl N/A BigDye v3.1
Click here to open a detailed view of this record 186 12-Oct-11 07-May-12 Red blinking light on 3130 Instrument Problem Applied Biosystems 3130/3130xl N/A N/A
Click here to open a detailed view of this record 185 22-Jul-11 23-Jul-11 Good sequence from 65mer primer with phosphorothioate Other Applied Biosystems 3730/3730xl PCR product BigDye v3.1
Click here to open a detailed view of this record 182 11-Jul-11 14-Jul-11 T peaks shifted forwards ! Please Help ! Applied Biosystems 3730/3730xl genomic DNA BigDye v3.1
Click here to open a detailed view of this record 181 04-Jul-11 14-Jul-11 comparison  of cleanup kits Other Applied Biosystems 3730/3730xl plasmid BigDye v3.1
Click here to open a detailed view of this record 180 23-Jun-11 01-Jul-11 Low RPA (signal intensity) ! Please Help ! Applied Biosystems 3130/3130xl plasmid BigDye v3.1
Click here to open a detailed view of this record 179 16-Jun-11 21-Jun-11 Baseline is high and colors separated ! Please Help ! Applied Biosystems 3730/3730xl plasmid BigDye v3.1
Click here to open a detailed view of this record 178 25-May-11 30-May-11 Short reads/ Signal falls off / Not well-defined peaks ! Please Help ! Applied Biosystems 3730/3730xl PCR product BigDye v3.1
Click here to open a detailed view of this record 177 18-May-11 30-May-11 Strong stop after Sample Problem Applied Biosystems 3730/3730xl plasmid BigDye v3.1
Click here to open a detailed view of this record 176 26-Apr-11 13-May-11 plasmid sequence problem Sample Problem Applied Biosystems 3100 plasmid BigDye v3.1
Click here to open a detailed view of this record 175 16-Apr-11 16-Jun-11 I get NNNNN as the final sequence ! Please Help ! Applied Biosystems 3730/3730xl PCR product BigDye v3.1
Click here to open a detailed view of this record 174 13-Apr-11 02-May-11 Templates for BigDye Direct? Other Applied Biosystems 3730/3730xl plasmid BigDye v3.1
Click here to open a detailed view of this record 173 15-Mar-11 20-Mar-11 Will sticky ends result in an "averaged" sequence? Sample Problem Applied Biosystems 3730/3730xl plasmid BigDye v3.1
Click here to open a detailed view of this record 172 10-Mar-11 20-Mar-11 different identities for the same nucleotide if sequenced forward or reverse ! Please Help ! Other-capillary plasmid N/A
Click here to open a detailed view of this record 171 25-Feb-11 14-May-11 Low signal  in 3130xl ! Please Help ! Applied Biosystems 3130/3130xl PCR product BigDye v3.1
Click here to open a detailed view of this record 170 25-Feb-11 14-May-11 3130xl low signal ! Please Help ! Applied Biosystems 3130/3130xl PCR product BigDye v3.1
Click here to open a detailed view of this record 169 19-Jan-11 20-Jan-11 Aliquoting POP7 ! Please Help ! Applied Biosystems 3730/3730xl plasmid BigDye v3.1
Click here to open a detailed view of this record 168 13-Jan-11 06-Mar-11 T peaks shifted forward ! Please Help ! Applied Biosystems 310 PCR product BigDye v3.1
Click here to open a detailed view of this record 167 11-Jan-11 13-Jan-11 No reads with abrupt peaks Instrument Problem Applied Biosystems 3130/3130xl plasmid BigDye v3.1
Click here to open a detailed view of this record 166 16-Dec-10 12-Jan-11 The shortest distance from the primer for a reliable base call? Other Applied Biosystems 3730/3730xl plasmid BigDye v3.1
Click here to open a detailed view of this record 165 19-Oct-10 14-May-11 3730XL Signal Decline ! Please Help ! Applied Biosystems 3730/3730xl plasmid DYEnamic ET Terminator
Click here to open a detailed view of this record 164 30-Sep-10 11-Oct-10 Gel Loading Dye in Sample ! Please Help ! Applied Biosystems 3730/3730xl PCR product BigDye v3.1
Click here to open a detailed view of this record 163 14-Sep-10 12-Jan-11 extra peaks ! Please Help ! Applied Biosystems 3730/3730xl PCR product BigDye v3.1
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