| DSRG Sequencing Troubleshooting Web Resource |
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Blue Veil |
In the gel image, appears as a blue haze migrating downward like a veil |
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Dye Blobs |
Bright fluorescence
superimposed on sequence, caused by the inefficient removal of unincorporated
dye terminators post-PCR. |
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G-Stop |
In the trace file view, observed as an abrupt termination of signal following a run of Gís. |
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Mobility Error |
Wrong mobility file applied, causing base peaks to appear shifted particularly at the start of the sequence. |
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Multiple Priming Sites |
Observed as multiple sequences/peaks superimposed, often due to extension products being generated from multiple priming sites |
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Red Rain |
From the gel image, appears as red streaks migrating down from the top of the screen |
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Slippage |
Polymerase slippage during PCR amplification and/or cycle sequencing due to a homopolymeric region in the template. Electropherogram may show a stutter or sawtooth pattern |
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Spike |
Large sharp peaks in data, may be one-color or four-color. |
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Trailing Signal |
Signal intensity decreases, appearing as gradually decreasing peak heights |
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Waterfall |
This refers to the raw data baseline. A raised baseline in all colors is observed at the start, which then drops after a given amount of time in a waterfall pattern. There may be a signal dropout visible in the electropherogram at this point. |


