The Light Microscopy Research Group and Corporate Relations Committee organized a conversation between light microscopy user groups and corporate partners, including Leica, Nikon, Olympus and Zeiss. This conversation provided an opportunity for each company to explain how they are being impacted by the COVID-19 pandemic and how they are adapting to the evolving needs of their customers.
Date: 11/18/2020
Time: 1:00-2:30 EST
View session recording.
Participants:
Ben Abrams, ABRF Light Microscopy Research Group and Corporate Relations Committee member, Director - Life Science Microscopy Facility Univ. of California Santa Cruz
Rich Cole, ABRF 2020 President, Light Microscopy Research Group member, Director - Advanced Light Microscopy & Image Analysis Core, New York State Dept. of Health Wadsworth Center
Joshua Rappoport, ABRF Light Microscopy Research Group member, Executive Director - Research Infrastructure at Boston College, Secretary – Core Technologies for Life Sciences
Michelle Itano, Director, UNC Neuroscience Microscopy Core Facility
Leica:
Greg Eppink General Manager Microscopy
Ryan Hrejsa, Senior Marketing Manager
Nikon:
Mike Gallo, General Manager, Service
Mike Johnson, Senior Regional Manager, Sales
Lynne Chang, Senior Marketing Manager
Olympus:
Shane Andrews, Manager, Sales Research Market, Life Science, Olympus Corporation of the Americas Scientific Solutions Group
Kerry Israel, Manager, Marketing Communications, Life Science, Olympus Corporation of the Americas Scientific Solutions Group
Zeiss:
Joseph Huff, Head of Marketing, North America, Zeiss Research Microscopy Solutions
Rosey Manser, Head of Business Development for Core Facilities
Studies
Activities
1) | ABRF2014 LMRG Group and CCMA Travel Award Winners - Richard Cole, John Russ, and Claire Brown (2,464K) - CCMA Travel Award Winners (1,934K) |
2) | ABRF2013 LMRG Photo - ABRF2013 LMRG Photo |
3) | ABRF 12 RG group photo - 2012 Meeting Group Photo |
4) | ABRF 12 RG intro talk - LMRG 2012 Overview Talk |
5) | ABRF 12 RG data talk - Study #2 Presentation (7,190K) |
6) | Proteomics workshop-- Get on Your Way to Microproteomics with laser Microdissection - Proteomics - Sarah Baxter |
7) | Proteomics workshop-- Sample prep - Tissue Proteomics Sample Preparation Leica Talk |
8) | ABRF 11 LMRG group photo - View Document (3,315K) |
9) | ABRF 11 RG talk - View Document (13,284K) |
10) | ABRF 2010 talk - View Document (4,643K) |
11) | Talk for ABRF 09 - View Document (10,567K) |
Protocols
1) | This is a paper written by Cole and Brown and gives a lot of background on how to measure point spread functions, why you may want to measure them, how to prepare bead slides and then a detailed protocol for setting up the instrument and measuring and interpreting the PSF. This article should be looked at first before referring to the specific protocols for the individual microscope platforms. - Nature Protocols Paper (1,371K) |
2) | PSF Protocol Leica SP5 - Measuring the PSF with the 0.175 um green fluorescent bead sample. - PSF Protocol Leica SP5 |
3) | PSF Protocol Nikon A1 - Measuring the PSF with the 0.175 um green fluorescent bead sample. - PSF Protocol Nikon A1 |
4) | PSF Protocol Olympus FV1000 - Measuring the PSF with the 0.175 um green fluorescent bead sample. - PSF Protocol Olympus FV1000 |
5) | PSF Protocol Zeiss 510 - Measuring the PSF with the 0.175 um green fluorescent bead sample using the AIM software. - PSF Protocol Zeiss 510 |
6) | Spectral Accuracy Protocol Leica SP5 - A mirror slide will be provided and you will look at the reflections of the laser lines into the spectral detector to determine if the wavelength readouts are accurate. - Spectral Accuracy Protocol Leica SP5 |
7) | Spectral Accuracy Protocol Olympus FV1000 - A mirror slide will be provided and you will look at the reflections of the laser lines into the spectral detector to determine if the wavelength readouts are accurate. - Spectral Accuracy Protocol Olympus FV1000 |
8) | Spectral Accuracy Protocol Zeiss 710 - A mirror slide will be provided and you will look at the reflections of the laser lines into the spectral detector to determine if the wavelength readouts are accurate. Uses the ZEN software. - Spectral Accuracy Protocol Zeiss 710 (261K) |
9) | Spectral Accuracy Protocol Zeiss 510 - A mirror slide will be provided and you will look at the reflections of the laser lines into the spectral detector to determine if the wavelength readouts are accurate. Uses the AIM software. - Spectral Accuracy Protocol Zeiss 510 |
10) | Spectral Separation Accuracy Protocol Leica SP5 - Protocol for spectral separation testing of software un-mixing with double orange stained beads. - Spectral Separation Accuracy Protocol Leica SP5 - Bead Core Spectra Text File - Bead Ring Spectra Text File - Bead Core Spectra lsf File (12K) - Bead Ring Spectra lsf File (9K) |
11) | Protocol for testing the spectral separation accuracy of the Olympus FV1000. - Spectral Separation Protocol Olympus FV1000 (1,133K) |
12) | Study #1 Laser Stability, Alignment and Co-registration Protocol - Study #1 Laser Stability, Alignment and Co-registr |
13) | Improved GFP imaging in live cells - Improved GFP imaging in live cells |
Publications
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Microsc and Microanal. 2013 Dec;19(6):1653-68.
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Microsc Microanal. 2011 Aug;17(4):598-606.
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Nature Protocols 6 (2011):1929
Membership History
Member Name | Organization | Details |
---|---|---|
Pamela Scott Adams | Trudeau Institute | Ad hocEB Liaison: 02/09 - 03/10 |
Carol J Bayles | Cornell University | Member: 04/08 - 05/15 |
Claire M Brown | McGill University | Chair: 03/11 - 04/15 Member: 09/10 - 03/18 |
Lisa Cameron | Duke University | Member |
Richard Cole | Wadsworth Center | Chair: 03/08 - 03/11 |
Arnold M. Falick | HHMI-UC Berkeley | Ad hocEB Liaison: 03/08 - 02/09 |
Paul Furcinitti | UMass Medical School | Member: 05/13 - 12/14 |
Anne-Marie Girard | Center for Genome Research and Biocomputing, Oregon State University | Member: 02/10 - 05/15 |
William G Hendrickson | Univ. of Illinois, Chicago | EB Liaison: 09/13 - 04/14 |
Jessica Hornick | Northwestern University | Member through 12/22 |
Karen R Jonscher | University of Colorado Denver | Ad hocEB: 04/10 - 03/13 |
Gary Laevsky | Princeton University | Member: 01/17-04/19 |
Karen Martin | West Virginia University | Member: 12/08 - 05/15 |
George McNamara | U Miami | Member: 06/11 - 03/13 Ad hoc: 03/13 - 12/14 |
Kary Oakleaf | Molecular Probes, Life Technologies | Member: 06/13 - 12/14 |
Cynthia Opansky | Blood Center of Wisconsin | Member: 02/09 - 12/13 |
James Powers | Indiana University | Member through 12/22 |
Joshua Rappoport | Northwestern University | Member: 03/15-01/19 |
Katherine Schulz | Blood Center of Wisconsin | Member: 03/09 - 04/13 |
Robert F. Stack | Wadsworth Center NYSDOH | Member: 02/09 - 03/11 |
Marc Thibault | Ecole Polytechnique | Member: 06/11 - 03/14 |
Erika Wee | McGill University | Chair: 04/15 - 05/18 |
Frances Weis-Garcia | Memorial Sloan Kettering Cancer Center | EB Liaison: 05/14 - 04/15 |
Questions or interest in joining an ABRF research group? Contact us!