Mission
The mission of the Protein Sequencing Research Group (PSRG) is to design studies to assess techniques for determining the primary structure of protein termini that will allow participating laboratories to gauge their expectations and competence in performing these analyses.
Questions or interest in joining an ABRF research group? Contact us!
PSRG Guidelines:
The PSRG has the responsibility to regularly design and distribute samples to the ABRF membership following a reasonable time line decided mutually by the PSRG and the Executive Board of ABRF (EB). This includes sample design (EB approval required), sample preparation, test evaluation by PSRG members, sample distribution, data receipt and evaluation, oral and/or poster presentation at the ABRF or other international meeting (e.g., Protein Society or ASBMB), and finally, publication in a peer reviewed journal. The study should be designed for N – and/or C-terminal sequence analysis of proteins containing the 20 common amino acids plus (optionally) other post-translationally modified amino acids that are commonly found in proteins. Any technology that yields information about the termini of proteins may be used. The PSRG should be comprised of 6-8 members plus one EB Liaison whose purpose is to assist the research group and to facilitate communication with the EB. The PSRG should meet about once a month via a conference call to discuss the current sample and ensure continued progress. Once a year, one of the members will be chosen as chairperson in a fashion determined by the PSRG. The chair has the ultimate responsibility for ensuring the success of the RG and its studies, from initial design to the final publication. This includes communication with the EB, management office, JBT or other journal, chairs of the other protein research groups, ABRF membership, and the yearly ABRF meeting organizers. Every year, 2-3 members rotate off the RG and are replaced by new members who are nominated by the PSRG for approval by the EB. The PSRG should use this as a guideline but is not limited to it, either in order presented or type of sample. To help ensure that the PSRG meets the continuing needs of ABRF members, each sample submission form would contain a few survey questions to better gauge the kinds of samples that would be most useful to our members and to solicit suggestions for future studies.
David Wood, Saint Louis University, Chair
Hediye Bromage, New York University School of Medicine
Allis Chien, Stanford University
Greg Cavey, Innovative Mass Spectrometry, LLC
Robert English, Shimadzu Scientific Instruments
Brian Feild, Shimadzu Scientific Instruments
Xuemei Luo, UTMB - Galveston
Sara McGrath, U.S. Food and Drug Administration
Magnus Palmblad, Leiden University Medical Center, (EB liaison)
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PSRG 2015-2016 Study: |
2) | PSRG 2014-2015 Study: N-terminal sequencing of standard proteins by dimethyl labeling and bottom-up mass spectrometry - View Study Announcement (34K) - View Sample Insert (359K) |
3) | PSRG 2013-2014 Study: N-terminal sequencing of standard proteins by N-terminal labeling and mass spectrometry - View Study Announcement (30K) - View Sample Insert (237K) |
4) | PSRG 2012-2013 Study : Sample preparation and terminal sequencing of a protein mixture - View Document 2012-2013 Study Announcement - View Document 2012 Participant Results Survey |
5) | PSRG 2011 Study: Comparing sensitivity limits for N-terminal Sequencing Technologies - View PSRG 2011 Study Announcement - View PSRG 2011 Study Sample Insert (36K) |
6) | PSRG 2010 Study: Comparing Edman and MS Sequencing of a Monoclonal Antibody (study closed) - View Study Announcement (94K) - View Cover Letter/Instructions (20K) - View Google Doc's data reporting form and survey |
7) | ESRG 2009 Study: Comparing Edman and Mass Spectrometry Techniques for N-terminal Sequencing - ESRG 2009 Study Announcement - Cover Letter/Sample Instructions (15K) - Vendor ABRF Study Participation Guidelines (18K) - Data Sheet - Excel file for both Edman and MS Data |
8) | ESRG 2008 Study "Investigation into homopolymeric amino acid N-terminal sequence tags and their effects on automated Edman degradation" - ESRG 2008 Study Announcement - Cover Letter/Sample Instructions - Data Sheet (41K) - Vendor ABRF Study Participation Guidelines (18K) |
9) | ESRG 2007 Study "Procedures used to Determine the N-Terminal Sequence of a Blocked Protein" - ESRG 2007 Study Announcement - Cover Letter/Sample Instructions - Deblocking Cycle - Data Sheet (25K) |
10) | ESRG 2006 Study "Edman Sequencing as a Method for Polypeptide Quantitation" - ESRG 2006 Study Announcement |
11) | ESRG 2005 Study "Identification of Modified Amino Acids by Edman Sequencing" - Instructions for Filling Out the Datasheet - Sample Description Including Modified Amino Acids - Data Sheet (19K) |
12) | ESRG 2004 Study "Modified Amino Acids in Edman Sequencing" - Sample Description Including Modified Amino Acids |
13) |
ESRG 2004 Study Aid: Pages 10-12 from "Identification of Modified PTH-Amino Acids in Protein Sequence Analysis" 1st ed. compiled by Mark Crankshaw and Greg Grant
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Activities
1) | ESRG Newsletter Vol.1 No.1, - Report on the ESRG at ABRF 2008, Salt Lake City, Utah and a Tribute to Pehr Edman |
Electronic Posters
PSRG 2015 - ABRF Poster and Oral Presentations: N-terminal Identification of a Standard Protein at Low Picomole Levels by Dimethyl Labeling and Bottom-up Mass Spectrometry | |
PSRG 2014 - ABRF Poster and Oral Presentations: N-terminal Sequencing of Standard Proteins by N-terminal Labeling and Mass Spectrometry | |
PSRG 2013 - ABRF Poster and Oral Presentations: Results of the PSRG 2013 Study Year 2: Terminal Sequencing of Standard Proteins in a Mixture |
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PSRG 2012 - ABRF Poster and Oral Presentations: Results of the PSRG 2012 Study: Terminal Sequencing of Standard Proteins in a Mixture, Year 1 of the 2 Year Study | |
PSRG 2011 - ABRF Presntations: Results of the PSRG 2011 Study: SensitivityAssessment of Edman and Mass Spectrometric Terminal Sequencing of an Undisclosed Protein - PSRG 2011 Poster (4,387K) - PSRG 2011 Oral Presentation (9,213K) |
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PSRG 2010 Poster: Edman and Mass Spectrometric Terminal Sequencing of a Monoclonal Antibody |
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PSRG 2010 Oral Presentation: N-Terminal Sequencing of an Antibody |
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ESRG 2009 Poster: Comparison of Edman and Mass Spectrometry Techniques for N-terminal Sequencing |
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ESRG 2009 Oral Presentation. "N-Terminal Sequencing Techniques" | |
ESRG 2008 Oral Presentation. "Effects of a Homopolymeric Amino Acid Tag on Edman Degradation" |
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ESRG 2008 Poster. "Investigation into Homopolymeric Amino Acid N-terminal Sequence Tags and their Effects on Automated Edman Degradation" |
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ESRG 2007 Poster: "Procedures used to Determine the N-Terminal Sequence of a Blocked Protein" | |
ESRG 2007 Oral Presentation: "Procedures used to Determine the N-Terminal Sequence of a Blocked Protein" | |
ESRG 2006 poster: "Edman Sequencing as a Method for Polypeptide Quantitation" | |
ESRG 2006: Oral presentation: Edman Sequencing as a Method for Polypeptide Quantitation |
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ESRG 2005 "State of Edman Sequencing" Survey, used for both the poster and oral presentation at the ABRF meeting, Savannah, GA, February 5-8, 2005 | |
ESRG 2005 poster "Identification of Modified Amino Acids by Edman Sequencing" | |
ESRG 2005: Oral presentation: Modified amino acids by Edman sequencing | |
"ABRF ESRG 2004 Study: Modified Amino Acids in Edman Sequencing"; ESRG study poster presented at the 2004 ABRF meeting February 28-March 2 in Portland, Oregon. | |
"Modified Amino Acids in Edman Sequencing"; ESRG study results presented at the 2004 ABRF meeting February 28-March 2 in Portland, Oregon. | |
"ABRF-ESRG 2003: Analysis of a PVDF-Bound Known Protein with a Homogeneous Amino-Terminus" ; ESRG study poster presented at the 2003 ABRF meeting February 10-13 in Denver, Colorado. | |
Slides from tutorial, "Making the Most of Your Edman Sequence Data; A Primer on Data Calling, Analysis, Interpretation and Reporting" in pdf format, presented at the 2003 ABRF meeting February 10-13 in Denver, Colorado. | |
Slides from the 2003 ESRG Study oral presentation given at the 2003 ABRF meeting, Feb 10-13 in Denver, Colorado. | |
"ABRF-2002 ESRG, a Difficult Sequence: Analysis of a PVDF-Bound Known Protein With a Heterogeneous Amino-Terminus"; poster presented at the 2002 ABRF meeting March 10-12 in Austin, Texas. | |
Slides from ESRG 2002 Study oral presentation given at 2002 ABRF meeting March 10-12 in Austin, Texas | |
The first half of the tutorial session "N-terminal Edman sequencing sample preparation" slides in pdf format. | |
Second half of the tutorial session "N-terminal Edman sequencing sample preparation" slides in pdf format. | |
Here is the method briefs handout from the tutorial session. Please use these methods and references only as guides for you to develop similar methods more fully in your own labs, no liability to ABRF for their use. |
Membership History
Member Name | Organization | Details |
---|---|---|
Daniel Brune | Arizona State Univ | Chair: 02/05 - 02/06 Member: 02/06 - 04/07 |
Scott Buckel | Amgen, Inc. | Member: 01/01 - 03/03 |
Dr. Allis Chien | Stanford University | EB Liaison: 03/17 - 09/18 |
Richard G. Cook | Baylor Col of Med | Member: 01/01 - 03/04 |
J. M. Crawford | Yale Univ. Keck Biotech Res. Lab. | Member: 01/01 - 03/04 |
Dr Nancy D Denslow | Univ of Florida-Gainesville | Chair: 03/04 - 02/05 Member: 03/02 - 03/04 EB liaison: 02/05 - 02/09 |
David R. Dupont | Applied Biosystems | Member: 01/01 - 03/02 |
Dr Robert English | Univ. of Texas Medical Branch | Chair_(co-chair): 04/12 - 04/13 Member: 05/11 - 04/12 |
Dr Hediye Erdjument-Bromage | Sloan Kettering Inst | Member: 04/14 - 04/15 |
Joseph Fernandez | Rockefeller Univ | Chair: 01/01 - 03/02 Member: 03/02 - 03/03 |
Mr. Mark K. Garfield | NIAID/NIH | Member: 05/12 - 04/15 |
Brian Hampton | Universtiy of Maryland School of Medicine | Member: 02/05 - 04/07 Member: 02/08 - 02/09 Ad hoc: 02/09 - 04/10 Co-Chair: 04/07 - 02/08 |
Dr William G Hendrickson | Univ. of Illinois, Chicago | EB Liason: 03/13 - 04/14 |
Peter Hunziker | University of Zurich | Chair: 02/08 - 02/09 MemberBecome Ad Hoc: 02/09 - 04/10 Member: 03/06 - 02/08 Ad hoc: 04/10 - 04/11 |
Pegah R Jalili | Sigma-Aldrich | Member: 06/11 - 04/14 |
Viswanatham Katta | Genentech | Member: 06/10 - 03/13 |
Ryuji Kobayashi | UT MD Anderson Cancer Center | Member: 03/03 - 03/06 |
William S. Lane | Harvard University | EB Liason: 10/03 - 02/05 |
Joseph W. Leone | Pfizer | Chair: 02/06 - 04/07 Member: 03/04 - 02/06 Member chair-emeritus: 04/07 - 02/08 |
Joseph F. Leykam | Michigan State University | Member: 04/07 - 08/07 |
Dr. Klaus D. Linse | Biosynthesis Inc. | Member: 02/05 - 02/08 |
Benjamin J Madden | Mayo Clinic | Chair: 02/03 - 03/04 Member: 03/04 - 02/05 Member: 01/01 - 02/03 |
Kwasi G Mawuenyega | Washington University School of Medicine | Member: 05/09 - 04/12 |
Dr. Sara C. McGrath | US FDA | Chair_(co-chair): 04/13 - 04/15 Member: 05/12 - 03/13 |
Dr. Ejvind Mortz | Alphalyse | : 03/13 - 04/14 |
John M Neveu | Harvard University | Chair: 04/02 - 01/03 Member: 01/01 - 03/02 Member: 02/03 - 02/05 |
Dr. Ronald L. Niece | Research Resources & Technologies | Member: 03/88 - 03/91 |
Dr. Brett S Phinney | Proteomics Core UC Davis Genome Center | EB Liason: 04/14 - 04/15 |
Dr Jan Pohl | Emory Univ Sch of Med | Member: 03/04 - 04/07 |
Dr. Henriette A. Remmer | University of Michigan | Member: 06/10 - 04/11 Ad hoc: 03/13 - 04/15 co-chair: 04/11 - 04/12 Co-chair: 04/12 - 03/13 |
Wendy Sandoval | Genentech, Inc. | Member: 04/10 - 04/11 Member changing to cochair: 04/07 - 02/09 Co-chair: 02/09 - 04/10 |
Jack Simpson | US Pharmacopeia | EB liaison: 02/09 - 03/13 |
John S. Smith | Univ of Texas Med Branch | Member: 04/10 - 04/11 Member changing to cochair: 09/07 - 02/09 Co-chair: 02/09 - 04/10 |
Laurey Steinke | University of Nebraska Medical Center | EB liason: 01/01 - 09/03 |
Dr. Detlev Suckau | Bruker Daltonik GmbH | MemberBruker Daltonics: 05/10 - 03/13 |
Richard S Thoma | Monsanto | Member: 02/08 - 02/09 Member: 02/05 - 04/07 Ad hoc: 02/09 - 04/10 Co-Chair: 04/07 - 02/08 |
James Walters | Sigma-Aldrich | Chair: 04/10 - 04/11 Member: 04/09 - 04/10 Ad hoc: 04/12 - 03/13 co-chair: 04/11 - 04/12 |
Dr. Frances Weis-Garcia | Memorial Sloan Kettering Cancer Center | EB Liaison: 02/16 - 03/17 |
Ken R. Williams | Yale Univ, Keck Lab | Member: 03/88 - 03/90 |
Dr. Bosong Xiang | Monsanto Company | Member: 04/09 - 04/12 |