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Journal of Biomolecular TechniquesDNA from Buccal Swabs Suitable for High-Throughput SNP Multiplex AnalysisWe sought a convenient and reliable method for collection of genetic material that is inexpensive and noninvasive and suitable for self-collection and mailing and a compatible, commercial DNA extraction protocol to meet quantitative and qualitative requirements for high-throughput single nucleotide polymorphism (SNP) multiplex analysis on an automated platform. Buccal swabs were collected from 34 individuals as part of a pilot study to test commercially available buccal swabs and DNA extraction kits. ....Read more A Pair of Ligation-Independent Escherichia coli Expression Vectors for Rapid Addition of a Polyhistidine Affinity Tag to the N- or C-Termini of Recombinant Proteins6×His tag is one of the most widely used affinity fusion tags that facilitates detection and purification of recombinant proteins. However, the location of this tag within a particular type of protein may influence the expression, solubility, and bioactivity of the protein, and the optimal location needs to be determined experimentally. To provide a tool for rapid generation of 6× His tags at the N- or C-terminus of any recombinant protein, we have constructed a pair of Escherichia coli expression vectors—pLIC-NHis and pLIC-CHis—based on the pET30a vector, for ligation-independent cloning (LIC)...Read more Isolation of Bothrops jararaca Snake Antithrombin from the Supernatant of Fibrinogen PurificationA novel method of antithrombin (AT) purification from Bothrops jararaca snake plasma was developed to obtain this protein using a waste supernatant from B. jararaca fibrinogen purification. The AT purification was achieved by affinity chromatography on HiTrap Heparin HP. The results showed an efficient purification process yielding pure AT (purity 65-fold and specific activity 368.91)... Read more ABRF Discussion ForumFeb 5, 2010, by Paul ConnollyNeed to analyze medical marijuana Feb 5, 2010, by "Moore, Helen (NIH/NCI) [E]"ARRA Funding Opportunity: NCI caHUB Acquisition of Normal Tissues Feb 4, 2010, by mark.wingerd@att.netCompatibility of tetrahydrofuran (THF) in nanoACQUITY UPLC system ABRF NewsABRF 2010: "Translating Basic Research With Advances in Biomolecular Technology"Registration for the ABRF2010 meeting is now open! National Science Foundation Educational Workshop Scholarships!The NSF, in partnership with the ABRF and Nemours, is sponsoring up to 25 Satellite Educational Workshop Awards at ABRF2010 to promote participation in this acclaimed series of technology-focused workshops. Deadline for applications is February 8th. Dr. Patrick Brown, Stanford University, has been chosen as this year's ABRF Award WinnerPatrick Brown has been selected as the 2010 ABRF Award winner, in recognition of his pioneering work in the development of microarrays, and the diverse applications of this technology in genetic research. ABRF/FASEB Career Fair and Employment Service at ABRF2010There will be a Job Fair and Employment Service available to attendees of this year’s meeting in Sacramento.This is a FREE service for job-seekers. Click "Read More" for more details! 2010 NCRR announcement for Shared Instrumentation GrantsPAR-10-082 (2010 SIG awards announcement) is now posted. A new maximum award of $600,000 has been set, and approximately 125 awards will be funded. Deadline for applications is 3/23/10. ABRF Executive Board Results Are In!The ABRF is pleased to announce the election of our two new EB members, David Friedman and Tom Neubert! NARG 2010 study on qRT-PCRThis is a benchmarking opportunity on Optimal Priming Strategies for cDNA Synthesis in Real-Time Quantitative PCR, cumulating the NARG community experience in the recent years Five exciting pre-meeting Educational Workshops announced for ABRF 2010 * Proteomics Platforms, * Targeted Proteomics, * RNA ABCs, * NextGen DNA Sequencing Analysis, and * Lab Management Skills.
Glycoprotein Research Group (gPRG) 2010 Study now openThe
Glycoprotein Research Group (gPRG)
would like to invite you to participate in a study that explores the use of
different approaches for determining the relative differences in N-glycosylation between
three similar glycoprotein samples. PSRG 2010 Study Announcement!The Protein Sequence Research Group invites you to participate in a study to
investigate both traditional and alternative methods for obtaining N-terminal information on a
complex mixture. Upcoming Meetings of InterestMar. 20-23, 2010ABRF 2010Sacramento, CA 2010 International Conference on Bioinformatics, Computational Biology, Genomics and Chemoinformatics (BCBGC-10)the Imperial Swan Hotel & Suites, 7050 South Kirkman Road, Orlando, Florida, United States 24th Annual Symposium of The Protein SocietyLooking at Proteins: Expanding Perspectives and New Technologies Manchester Grand Hyatt, San Diego, CA, United States |
